SOLUTION OF SPOROPOLLENIN AND REAGGREGATION OF A SPOROPOLLENIN-LIKE MATERIAL - A NEW APPROACH IN THE SPOROPOLLENIN RESEARCH

Exines from Typha angustifolia L. pollen were completely dissolved in 2-aminoethanol if the exine material was isolated and purified by an e nzymatic procedure. Acetolysis or treatment of the exine material with H3PO4 before the dissolution process led to insolubility. A reaggrega tion of the dissolved material was possible when the solvent was remov ed and H2O added. The initial exine material and the reaggregated mate rial were compared by means of staining, fluorescence microscopy, SEM, resistance against acetolysis, DSC, FTIR and C-13 CP/MAS NMR spectros copy. Both materials showed great similarities with only little differ ences in the FTIR and C-13 NMR spectra and in the DSC thermograms.