Dwm. Cook et al., DEVELOPMENT OF AN APPARATUS AND PROTOCOL FOR THE EFFICIENT ISOLATION OF BACTERIA AND YEASTS THAT ATTACH TO BOTRYTIS-CINEREA GERMLINGS, Annals of Applied Biology, 131(2), 1997, pp. 213-226
An apparatus and protocol for the efficient and consistent isolation o
f bacteria and yeasts with the ability to attach to germlings of Botry
tis cinerea is described. The study focused on minimising microbial co
ntamination by bacteria or yeasts which do not attach to the pathogen
B. cinerea but which interact with materials used in the equipment and
would otherwise be isolated along with target microbes. After develop
ment, the assay reduced the contamination rate to 1-2 cells per 100 ad
ded and this was found to be a satisfactory level for the selection of
microbial attachers to fungal hyphae. One or more phenotypes of micro
bes that adhered to B. cinerea germlings were found in 97% of the 70 s
amples collected from phylloplane washings and processed using this as
say.