MOLECULAR PHYLOGENY OF MANGROVES-III PARENTAGE ANALYSIS OF A RHIZOPHORA HYBRID USING RANDOM AMPLIFIED POLYMORPHIC DNA AND RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM MARKERS

In this study, random amplified polymorphic DNA (RAPD) markers of geno mic DNA and restriction fragment length polymorphism (RFLP) of mitocho ndrial DNA using a mitochondrial genome specific probe, atp 6 (from ma ize), were used to identify the parentage of a Rhizophora hybrid, The combined RAPD profiles of two species, Rhizophora apiculata and R. muc ronata had 96.5% similarity with that of the hybrid, indicating that t hese two species are the probable parents, RFLPs for the mitochondrial genome were readily observed, and the RFLP profile of R. apiculata wa s exactly the same as that of the hybrid, establishing its maternal st atus for the hybrid. This is the first report on characterising the pa rentage of a Rhizophora hybrid using molecular markers. (C) 1997 Elsev ier Science B.V.