CCD VIDEO OBSERVATION OF MICROGRAVITY CRYSTALLIZATION OF LYSOZYME ANDCORRELATION WITH ACCELEROMETER DATA

Citation
Eh. Snell et al., CCD VIDEO OBSERVATION OF MICROGRAVITY CRYSTALLIZATION OF LYSOZYME ANDCORRELATION WITH ACCELEROMETER DATA, Acta crystallographica. Section D, Biological crystallography, 53, 1997, pp. 747-755
Citations number
28
ISSN journal
09074449
Volume
53
Year of publication
1997
Part
6
Pages
747 - 755
Database
ISI
SICI code
0907-4449(1997)53:<747:CVOOMC>2.0.ZU;2-R
Abstract
Lysozyme has been crystallized using the ESA Advanced Protein Crystall ization Facility onboard the NASA Space Shuttle Orbiter during the IML -2 mission. CCD video monitoring was used to follow the crystallizatio n process and evaluate the growth rate. During the mission some tetrag onal crystals were observed moving over distances of up to 200 mu m. T his was correlated with microgravity disturbances caused by firings of vernier jets on the Orbiter. Growth-rate measurement of a stationary crystal (which had nucleated on the growth reactor wall) showed spurts and lulls correlated with an onboard activity: astronaut exercise. Th e stepped growth rates may be responsible for the residual mosaic bloc k structure seen in crystal mosaicity and topography measurements.