CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF THE SACCHAROMYCOPSIS-FIBULIGERA GLUCOAMYLASE EXPRESSED FROM THE GLU1 GENE IN ESCHERICHIA-COLI

Authors
SOLOVICOVA A GASPERIK J SEVCIK J HOSTINOVA E
Citation
A. Solovicova et al., CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF THE SACCHAROMYCOPSIS-FIBULIGERA GLUCOAMYLASE EXPRESSED FROM THE GLU1 GENE IN ESCHERICHIA-COLI, Acta crystallographica. Section D, Biological crystallography, 53, 1997, pp. 782-783
Citations number
12
Journal title
Acta crystallographica. Section D, Biological crystallography → ACNP
ISSN journal
09074449
Volume
53
Year of publication
1997
Part
6
Pages
782 - 783
Database
ISI
SICI code
0907-4449(1997)53:<782:CAPAOT>2.0.ZU;2-Y
Abstract
The active non-glycosylated glucoamylase, overexpressed from the Sacch aromycopsis fibuligera GLU1 gene in Escherichia coil BL21(DE3), has be en purified from the solubilized inclusion bodies and then renatured i n vitro. Crystals of the recombinant glucoamylase were obtained by vap our diffusion using PEG as precipitant. The crystals belong to the ort horhombic space group P2(1)2(1)2(1), with unit-cell dimensions of a = 58.1, b = 87.8 and c = 99.9 Angstrom, and diffract to 1.7 Angstrom res olution. This is the first report of the crystallization of the full-l ength glucoamylase corresponding to the mature enzyme.