A. Solovicova et al., CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF THE SACCHAROMYCOPSIS-FIBULIGERA GLUCOAMYLASE EXPRESSED FROM THE GLU1 GENE IN ESCHERICHIA-COLI, Acta crystallographica. Section D, Biological crystallography, 53, 1997, pp. 782-783
The active non-glycosylated glucoamylase, overexpressed from the Sacch
aromycopsis fibuligera GLU1 gene in Escherichia coil BL21(DE3), has be
en purified from the solubilized inclusion bodies and then renatured i
n vitro. Crystals of the recombinant glucoamylase were obtained by vap
our diffusion using PEG as precipitant. The crystals belong to the ort
horhombic space group P2(1)2(1)2(1), with unit-cell dimensions of a =
58.1, b = 87.8 and c = 99.9 Angstrom, and diffract to 1.7 Angstrom res
olution. This is the first report of the crystallization of the full-l
ength glucoamylase corresponding to the mature enzyme.