TRANSITION OF SERUM VITELLOGENIN CYCLE IN SAKHALIN TAIMEN (HUCHO-PERRYI)

A specific and sensitive enzyme linked immunosorbent assay (ELISA) and a single radial immunodiffusion (SRID) were developed for measurement of serum vitellogenin (Vg) levels in Sakhalin taimen (Hucho perryi). Regarding specificity for serum Vg, an antiserum raised against lipovi tellin of taimen (a-Lv) was adequate for both assays. ELISA and SRID c ould detect Vg in serum at concentrations as low as 10 ng/ml and 25 mu g/ml, respectively. In estrogen administration experiments, the level of serum Vg began clearly increasing within 12 to 24 hr after injecti on of immature females with estradiol-17 beta (E-2) The appearance and levels of Vg in males treated with E-2 were delayed and smaller, resp ectively, than for females. Vg levels varied throughout natural vitell ogenesis from 0-4 mu g/ml (3 years old) to approximately 30 mg/ml (5-6 years old). We observed an early transitory peak of serum Vg levels ( primary reaction) at the time of early vitellogenesis and chronic high Vg levels (for 6-7 months) in winter period before ovulation. Changes of serum E-2 levels were correlated with Vg levels. However, E-2 leve ls decreased a month earlier than Vg levels near ovulation. It appears that the duration of vitellogenesis in taimen is considerably longer than that in other salmonids, lasting more than 2 years. (C) 1997 Else vier Science Inc.