S. Fukuoka et al., ELUCIDATION OF THE STRUCTURE OF THE CORE REGION AND THE COMPLETE STRUCTURE OF THE R-TYPE LIPOPOLYSACCHARIDE OF ERWINIA-CARATOVORA FERM P-7576, European journal of biochemistry, 250(1), 1997, pp. 55-62
An R-type lipopolysaccharide (LPS) from Erwinia carotovora strain FERM
P-7576 was studied after strong alkaline degradation and mild acid hy
drolysis. The resulting products were analysed by fast-atom bombardmen
t mass spectrometry, one-and two-dimensional H-1 and C-13 NMR spectros
copy, dephosphorylation and methylation analysis. The following struct
ure was proposed for the core region of the LPS: [GRAPHICS] where Hep
is L-glycero-D-manno-heptose and Kdo is 3-deoxy-D-manno-octulosonic ac
id. Some LPS species lack the beta-D-Galp residue or the beta-D-Galp-(
1-7)-alpha-Hepp disaccharide. With the known structures of lipid A [Fu
kuoka, S., Kamishima, H., Nagawa, Y., Nakanishi, H., Ishikawa, K., Niw
a, Y., Tamiya, E. & Karube, I. (1992) Arch. Microbiol. 157, 311-318] a
nd the core moiety, the complete LPS structure was established and con
firmed by matrix-assisted laser desorption/ionisation time-of-flight m
ass spectrometry of the native and O-dcacylated LPS.