MICROPROPAGATION OF SATUREJA-OBOVATA LAG

A micropropagation procedure for juvenile and adult savory (Satureja o bovata Lag.) explants is described. Pretreatment of the nutlets with g ibberellic acid (0.57 mm) did not improve in vitro germination. Optimu m shoot proliferation of juvenile and adult material was obtained on m edium containing 2.22 mu M N-6-benzyladenine. Rooting and acclimatizat ion of juvenile shoots were accomplished in vivo, while adult shoots w ere rooted in vitro after 3 days of exposure to 4.92 mu M indole-3-but yric acid followed by subsequent transfer to auxin-free medium. More t han 95% survival of adult rooted plants was obtained during the acclim atization phase. Chemical names used: gibberellic acid (GA(3)); N-6-be nzyladenine (BA); indole-3-butyric acid (IBA); isopentenyladenine (2iP ).