REGULATION OF SERTOLI-CELL IL-1 AND IL-6 PRODUCTION IN-VITRO

Interleukin-1 (IL-1) and IL-6 are pleiotropic cytokines produced by a large variety of cell types. In the testis, Sertoli cells produce IL-1 alpha and IL-6. Previous studies have demonstrated that, in vitro, Se rtoli cell IL-1 alpha production is stimulated by some inducers of mac rophage IL-1, as well as by phagocytosis of residual bodies. Furthermo re, we have also shown that IL-lcr is able to enhance Sertoli cell IL- 6 production by an autocrine action. The aim of the present study was to further investigate the regulation of Sertoli cell IL-1 and IL-6 pr oduction. Three categories of potential regulators were tested; the li popolysaccharide (LPS) and the yeast extract zymosan; follicle stimula ting hormone (FSH), testosterone and dexamethasone; tumor necrosis fac tor alpha (TNF alpha), interferon gamma (IFN gamma) and the nerve grow th factor beta (NGF beta). It was found that zymosan (400-800 mu g/ml) and LPS (20 mu g/ml) stimulated Sertoli cell IL-1 and IL-6 production . FSH (1 x 10(-2)-1 mu g/ml) and NGF (25-200 mu g/ml) stimulated Serto li cell IL-6 levels in a dose-dependent manner but had no effect on IL -1. The effect of testosterone on Sertoli cell IL-1 and IL-6 secretion was biphasic: dramatic increased secretion with low concentrations (0 .01-1 nM) and no effect with the higher concentration tested (100 nM). Dexamethasone reduced LPS-induced IL-1 and IL-6 production in a conce ntration-responsive manner (0.04-0.4 and 0.4-40 ng/ml, respectively). Addition of TNF alpha to Sertoli cells resulted in a dose-dependent in crease of both cytokines (50-100 U/ml for IL-1, 100-200 U/ml for IL-6) . In the case of IFN gamma, intermediate concentrations (50-100 U/ml) stimulated IL-1 alpha, whereas the highest concentrations (200-400 U/m l) inhibited IL-6. It is concluded that regulation of Sertoli cell IL- 1 and IL-6 is very complex as it involves factors as different as horm ones, paracrine factors and activators of macrophages. The latter agen ts may be mimicking the action of pathogens or the action of intratest icular agents whose nature remains to be elucidated. (C) 1997 Elsevier Science Ireland Ltd.