HADAMARD 1D H-1 TOCSY AND ITS APPLICATION TO OLIGOSACCHARIDES

Replacement of a series of N consecutive one-site selective excitation NMR experiments by N simultaneous N-site excitations with phases of c omponent pulses varied according to the N x N Hadamard matrix offers a sensitivity improvement of the order of root N (for N = 2, 4, 8,..,) [R. Freeman, Spin Choreography, pp. 177-183. Spektrum, Oxford (1996)]. Here this principle was extended to one-dimensional (1D) H-1 TOCSY ex periments, in the absence and presence of heteronuclear decoupling, Th e 1D H-1 Hadamard TOCSY technique was applied to selected oligosacchar ides, representing a class of biomolecules for which multiple 1D TOCSY experiments are the mainstay in H-1 NMR spectral assignment and struc tural characterization. (C) 1997 John Wiley & Sons, Ltd.