NEURONAL DYE-COUPLING IN THE DEVELOPING RAT FASCIA-DENTATA

The present study describes dye coupling among neurons of the developi ng rat fascia dentata following impalement and intracellular filling w ith Neurobiotin. The number of neuronal impalements resulting in dye-c oupled cells decreases from P14 to P120. The most rapid decline in dye coupling was observed between P14 and P21, the beginning of the most active period of synaptogenesis in the dentate molecular layer. Dye co upling between granule cells and axo-axonic interneurons (chandelier c ells) accounts for about 10% of the dye-coupled neuronal population ac quired in slices from P14 and P21 rats and declines to less than 5% by P60 and P120. Our data suggest that dye coupling is related reciproca lly to the number of synapses formed on granule cells. Thus the relati onship of dye coupling to synaptic density in the developing fascia de ntata is similar to that reported in studies of the aging fascia denta ta. Also the observation of axo-axonic interneurons coupled to granule cells at all ages suggests an interesting neuronal arrangement with t he potential of limiting granule cell discharge to discrete neuronal a ssemblies in response to perforant path input. (C) 1997 Elsevier Scien ce B.V.