YEAST MICROARRAYS FOR GENOME WIDE PARALLEL GENETIC AND GENE-EXPRESSION ANALYSIS

We have developed high-density DNA microarrays of yeast ORFs. These mi croarrays can monitor hybridization to ORFs for applications such as q uantitative differential gene expression analysis and screening for se quence polymorphisms. Automated scripts retrieved sequence information from public databases to locate predicted ORFs and select appropriate primers for amplification, The primers were used to amplify yeast ORF s in 96-well plates, and the resulting products were arrayed using an automated micro arraying device. Arrays containing up to 2,479 yeast O RFs were printed on a single slide. The hybridization of fluorescently labeled samples to the array were detected and quantitated with a las er confocal scanning microscope. Applications of the microarrays are s hown for genetic and gene expression analysis at the whole genome leve l.