QUANTITATIVE-ANALYSIS OF CORN ZEINS BY CAPILLARY ELECTROPHORESIS

Kjeldahl analysis is commonly used to measure zein proteins in corn ma ize (N x 5.7) with no attempt to eliminate contribution from other nit rogen sources. In this study, dry milled corn was extracted with 70% e thanol or 0.1N NaOH and the zein content of the extract measured using capillary electrophoresis in the presence of sodium dodecyl sulfate. The amount of zein protein in alcohol extracts, using this method, was in good agreement with that determined by Kjeldahl nitrogen analysis. However, less than half of the Kjeldahl nitrogen in the alkaline extr acts could be attributed to corn zein. Reproducibility expressed as re lative standard deviation for migration time and peak area was 0.10 an d 1.05, respectively The technique permits rapid analysis of a large n umber of samples without interference from other compounds present in the extracts.