SERIAL CYTOKINE CHANGES IN PERITONEAL EFFLUENT AND PLASMA DURING PERITONITIS IN PATIENTS ON CONTINUOUS AMBULATORY PERITONEAL-DIALYSIS (CAPD)

Objective and Design: Secretion of IL-2 and sIL-2R in the peritoneal d ialysis fluid and in the peripheral blood during peritonitis with refe rence to the process of IL-6 and IL-8 release were investigated. Subje cts: 17 patients with end-stage renal disease, treated with continuous ambulatory peritoneal dialysis. Methods: ELISA method using commercia l kits, Genzyme Corp Boston and DAKO. Results: Markedly increased IL-6 (mean; 2895+/-1360 pg/ml) and IL-8 concentration (1459+/-966 pg/ml) i n drain dialysate fluid at the onset of peritonitis, scarred to drop r apidly in the successive samples after antibiotics had been administer ed. Statistically significant increase of IL-2 (197+/-92 pg/ml) and sI L-2R (287+/-79 pg/ml) was observed 16h later and kept increasing until reaching the peak after 24 h. Conclusion: Secretion of IL-6 and IL-8 pro-inflammatory cytokines which are mainly synthesized in mesothelium cells is followed by the activation of lymphocytes, their infiltratio n and the production of T lymphocyte derived IL-2 and sIL-2R.