THE DETERMINATION OF PAMIDRONATE IN PHARMACEUTICAL PREPARATIONS BY ION-PAIR LIQUID-CHROMATOGRAPHY AFTER DERIVATIZATION WITH PHENYLISOTHIOCYANATE

An analytical method was developed for the determination of pamidronat e [(3-amino-1-hydroxypropylidene)bisphosphonate] by ion-pair liquid ch romatography. The analyte was derivatized with phenylisothiocyanate in to an UV-absorbing derivative. The reaction product was cleaned-up by a double ion-pair extraction and treated with hydrogen peroxide prior to injection. Both, the detection limit and the lower limit of quantif ication of pamidronate in water were 0.1 mu g ml(-1) disodium pamidron ate. The intra-day precision was 3% for a 5-mu g ml(-1) pamidronate st andard solution and the inter-day precision 6% for a 3-mu g ml(-1) sol ution. The method was applied in the quality control of pamidronate in jection concentrates and tablets. (C) 1997 Elsevier Science B.V.