INCREASED EXPRESSION OF AN ENDOPEPTIDASE (GAMMA-EGE IDE) HYDROLYZING BETA-ENDORPHIN DURING DIFFERENTIATION AND MATURATION OF BONE-MARROW MACROPHAGES/

Citation
B. Sarada et al., INCREASED EXPRESSION OF AN ENDOPEPTIDASE (GAMMA-EGE IDE) HYDROLYZING BETA-ENDORPHIN DURING DIFFERENTIATION AND MATURATION OF BONE-MARROW MACROPHAGES/, Journal of leukocyte biology, 62(6), 1997, pp. 753-760
Citations number
46
Categorie Soggetti
Immunology,Hematology
ISSN journal
07415400
Volume
62
Issue
6
Year of publication
1997
Pages
753 - 760
Database
ISI
SICI code
0741-5400(1997)62:6<753:IEOAE(>2.0.ZU;2-6
Abstract
The presence and regulated expression of peptidase activity is a power ful mechanism with the potential to terminate or alter receptor recogn ition, cell membrane signal transduction, and physiological responses of immune cells to exogenous opioid peptides, In this study, the expre ssion of an endopeptidase that hydrolyzes beta-endorphin to gamma-endo rphin and other peptide products was investigated during in vitro diff erentiation and maturation of recombinant ganulocyte-macrophage colony -stimulating factor (rGM-CSF) -derived, bone marrow-derived macrophage s. In freshly isolated intact isolated mouse bone marrow cells the rat e of beta-endorphin hydrolysis is undetectable (<0.1 nmol beta-endorph in hydrolyzed/h/10(6) cells), However, total intracellular beta-endorp hin hydrolytic activity was increased significantly to 20.0 +/- 1.7 nm ol/h/10(6) cells in the mature mouse macrophages derived in vitro by c ulture with rGM-CSF. rGM-CSF-derived macrophages expressed significant ly higher levels of both protein and mRNA for the major beta-endorphin endopeptidase, gamma-endorphin-generating enzyme/insulin-degrading en zyme (gamma-EGE/IDE), Moreover, this enzymatic activity appears to be responsible for cleavage of exogenous beta-endorphin by Intact rGM-CSF -derived macrophages or peritoneal macrophages to generate gamma-endor phin and other peptide products.