DERIVATIZATION REACTIONS FOR THE SPECTROFLUOROMETRIC DETERMINATION OF-(AMINO-SUBSTITUTED-PHENYL)-5H-2,3-BENZODIAZEPINES

Fluorescent derivatization reactions of five -(amino-substituted-pheny l)-5H-2,3-benzodiazepines were investigated. In the first (''A'') reac tion the 2,3-benzodiazepines can transform into 2-naphthol derivatives having intensive fluorescence in highly acidic medium by hydrolysis a nd subsequent aldol condensation in basic medium (with the exception o f yl)-4-methyl-7,8-dimethoxy-5H-2,3-benzodiazepine). In the second ('' B'') reaction the 2-naphthol derivatives are formed directly in dimeth ylsulfoxide (DMSO) solution in the presence of alkali-hydroxide by hea ting. With the use of these reactions sensitive simple and rapid spect rofluorimetric analytical methods were developed for the quantitative determination of Ave 2,3-benzodiazepine derivatives.