EFFECTS OF D-GLUCOSE ON CHEMOKINESIS AND RESTING PRODUCTION OF REACTIVE OXYGEN SPECIES IN NEUTROPHIL GRANULOCYTES OF LEAN OR OBESE-HYPERGLYCEMIC MOUSE

The response to D-glucose (0-21 mM) was studied in neutrophil granuloc ytes from obese, hyperglycemic and hyperinsulinemic Ume (a) over circl e ob/ob mice and their lean, littermate controls in order to further e lucidate the effects of in vivo and in vitro hyperglycemia on neutroph il function. Neutrophil random locomotion on glass and neutrophil rest ing luminol-enhanced chemiluminescence in cell suspension were studied . Random locomotion was stimulated by D-glucose in neutrophils from bo th Ume (a) over circle ob/ob and control mice but the locomotive activ ity in Ume (a) over circle ob/ob mouse neutrophils was significantly h igher than that found in the controls at 4-21 mM glucose. In both type s of mice, the stimulatory effect of D-glucose on random locomotion wa s diminished at 21 mM glucose (not significantly different from that a t 0 mM glucose). Resting chemiluminescence from mouse neutrophils was also stimulated by glucose but here the magnitude of response was simi lar in neutrophils from both types of mice. These results indicate tha t chronic hyperglycemia and hyperinsulinemia in the Ume (a) over circl e ob/ob mouse may be associated with an increased neutrophil random lo comotive activity but a similar resting production of reactive oxygen species, as compared with neutrophils from control mice at physiologic al and hyperglycemic glucose concentrations in vitro.