INVESTIGATION OF MALONDIALDEHYDE FORMATION AND ANTIOXIDANT ENZYME-ACTIVITY IN STORED-BLOOD

In the present study, fresh blood obtained from six healthy adult male donors was investigated for erythrocyte malondialdehyde (MDA) formati on and antioxidative enzyme activity. Plasma and erythrocyte membrane lipid peroxidation were measured by MDA formation. Erythrocyte superox ide dismutase (SOD) and glutathione peroxidase (GSH-Px) levels were de termined in whole blood stored for transfusion purposes. Erythrocyte a nd plasma MDA levels increased significantly during the storage period from day 3 to day 19 and, after that, stayed unchanged. Erythrocyte M DA increased 100% on day 7 when compared to day 1. The GSH-Px activity significantly decreased after day 9 and SOD decreased after day 13. T he reduction in enzyme activities continued until day 27. Our results showed significant alteration in erythrocyte membrane and plasma MDA f ormation and intracellular antioxidant enzyme status in whole blood us ed for transfusion. However, we do not know whether such alterations h ave clinical importances for the recipient.