MEASUREMENT OF CHEMILUMINESCENCE FROM NEUTROPHILS IN A 96-WELL MICROPLATE USING LUMI-BOX U-800-II

We have developed a microplate photon counting system based on a coole d charge-coupled device (Lumi Box U-800 II) jointly with Maikurotekku Nition Company (Chiba, Japan). The system makes it possible to quantif y chemiluminescence (CL) in a 96-well microplate automatically and sim ultaneously in a single experiment. We studied the measurement conditi ons for a luminol-dependent CL assay from neutrophils stimulated with opsonized zymosan (OZ) using this system. Parameters examined included the effect of OZ dose per well, mixing speed, mixing time and detecti on time on CL responses. The results indicated that this system allows the measurement of CL from phagocytes on a large number of samples us ing small amounts of sample and regents. (C) 1997 John Wiley & Sons, L td.