PLANT CDC2 IS NOT ONLY TARGETED TO THE PRE-PROPHASE BAND, BUT ALSO COLOCALIZES WITH THE SPINDLE, PHRAGMOPLAST, AND CHROMOSOMES

A polyclonal antiserum against the p34(cdc2) homologue of Arabidopsis thaliana, CDC2aAt, was used in parallel with a polyclonal antiserum ag ainst the PSTAIRE motif to study the subcellular localization of CDC2 during the cell cycle of isolated root tip cells of Medicago sativa, D uring interphase, CDC2 was located in the nucleus and in the cytoplasm , The cytoplasmic localization persisted during the complete cell cycl e, whereas the nuclear signal disappeared at nuclear envelope breakdow n, At the beginning of anaphase, the anti-CDC2aAt antibody transiently co-localized with condensed chromosomes. The chromosomal co-localizat ion disappeared as anaphase continued and remained excluded from the s eparated chromosomes until cytokinesis, when CDC2 re-located to the ne wly forming nuclei, We also observed a co-localization of CDC2 with th ree microtubular structures, the pre-prophase band, the spindle, and t he phragmoplast. (C) 1997 Federation of European Biochemical Societies .