A SULFATIDE RECEPTOR DISTINCT FROM L-SELECTIN IS INVOLVED IN LYMPHOCYTE-ACTIVATION

Sulfatide induces leukocyte activation, which is thought to be mediate d,ia L-selectin, Here we demonstrate that lymphocytes express a recept or for sulfatide distinct from L-selectin and that this receptor is in volved in sulfatide-induced cell activation, While cell surface L-sele ctin expression mas abolished by phorbol 12-myristate 13-acetate (PMA) , lymphocytes retained the ability to bind sulfatide in liquid phase a s well as in immobilized solid phase, The novel sulfatide receptor obt ained from PMA-treated lymphocytes showed a molecular size of 65 kDa, Stimulation through this receptor triggered cytosolic free Ca2+ elevat ion and intercellular aggregation. (C) 1997 Federation of European Bio chemical Societies.