SERUM LEVELS OF INTERLEUKIN-1-BETA, LUTEINIZING-HORMONE, AND PROLACTIN CORRELATE WITH THE EXPRESSION OF CD45 ISOFORMS ON CD4-BLOOD T-LYMPHOCYTES IN HEALTHY WOMEN( PERIPHERAL)

The expected association between age and the CD45 isoforms expression on CD4+ T-PBL is much more obvious in men than in women. We investigat ed whether or not circulating factors influence the differentiation of CD4+ T-PBL. Peripheral blood samples were obtained from 56 healthy ag e-matched subjects (28 men and 28 women, 21-55 years old). Mononuclear leukocytes were analyzed by three-color flow cytometry. The serum con centrations of interleukin-1 beta (IL-1 beta), interleukin-6, tumor ne crosis factor-alpha (TNF alpha), GM colony-stimulating factor, prolact in (Prl), and luteinizing hormone (LH) were determined by ELISA. The e xpected age-related decrease of naive (CD45RA+,RO-) cells and increase of memory (CD45RA-,RO+) cells among CD4+ T-PBL were observed in men o nly (p < 0.001 and 0.005). In women, these correlations were not signi ficant. On the other hand, in women only, elevated IL-1 beta was assoc iated with fewer naive and more memory cells among CD4+ T-PBL (p < 0.0 01). In both sexes, IL-1 beta correlated with the expression of CD25 o n CD4+ T-PBL (on either naive or memory cells, p < 0.001). Other cytok ines or the CD8+ T-PBL showed no significant correlation. In women, th e elevation of LH at mid-cycle inversely correlated with the proportio n of naive CD4+ T-PBL (p < 0.01). Elevated LH was associated with more CD25 on memory CD4+ T-PBL (p < 0.01). A significant correlation exist s between IL-1 beta and LH (p < 0.001). Furthermore, in both sexes, Pr l correlated with the proportion of CD4+ cells among T-PBL. In men, el evated Prl was associated with more naive CD4+ T-PBL (p<0.005), while in women, Prl correlated with more transient CD45RA+, RO+ cells among CD4+ T-PBL and increased TNF-alpha (p < 0.05 for both). Thus, circulat ing IL-1 beta could be involved in the expression of CD25 on CD4+ T-PB L and favors the generation of memory CD4+ T-PBL. In women, the IL-1 b eta- and/or mid-cycle-dependent processes seem to overwhelm the age-re lated changes. Elevated Prl might exert a dual influence: it favors th e development of naive CD4+ T lymphocytes and possibly acts in, synerg y with other cytokines during immune stimulation.