INVESTIGATION OF PEAK COMPRESSION EFFECTS IN PACKED-COLUMN SUPERCRITICAL-FLUID CHROMATOGRAPHY USING CHEMOMETRICS

Authors
CARLSSON D STRODE JT GYLLENHAAL O KARLSSON A KARLSSON L
Citation
D. Carlsson et al., INVESTIGATION OF PEAK COMPRESSION EFFECTS IN PACKED-COLUMN SUPERCRITICAL-FLUID CHROMATOGRAPHY USING CHEMOMETRICS, Chromatographia, 44(5-6), 1997, pp. 289-294
Citations number
20
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Chromatographia → ACNP
ISSN journal
00095893
Volume
44
Issue
5-6
Year of publication
1997
Pages
289 - 294
Database
ISI
SICI code
0009-5893(1997)44:5-6<289:IOPCEI>2.0.ZU;2-J
Abstract
In this paper chemometrics have been used to study and characterize pe ak compression phenomena in packed column SFC. A carbon dioxide/2-meth yl-1-propanol mobile phase was used in the experimental design (modifi er concentration, temperature and pressure) and modelling part of the investigation. A cubic interaction term was needed in the model to obt ain a reasonable fit, suggesting that all three parameters are of sign ificance in terms of controlling peak compression. At the optimum cond itions derived from the model a narrow peak was obtained as predicted.