A WEAK CATION-EXCHANGE PHASE FOR THE SEPARATION OF BIOGENIC-AMINES BYOPEN-TUBULAR LIQUID-CHROMATOGRAPHY

The preparation and performance of a weak cation-exchange stationary p hase for Open Tubular Liquid Chromatography (OT-LC) was investigated. The stationary phase was prepared in 5.4 mu m I.D. fused silica capill aries by in situ photopolymerization of a mixture of silicon acrylate and acrylic acid. The influence of pH, counter ion concentration and o rganic modifier concentration of the mobile phase on the retention was studied with catecholamines as test solutes using LIF detection. Othe r biological amines like amino acids, small peptides and nucleic acid derivatives could be separated on this stationary phase as well. The k inetic performance of the stationary phase was studied with several ca tions and neutral solutes.