NEUTRAL CYCLODEXTRINS AS CHIRAL AGENTS FOR ENANTIOMERIC SEPARATIONS OF CHROMANES IN CAPILLARY ELECTROPHORESIS

Six different cyclodextrins with varying cavity size and rim substitut ion were used as chiral agents for the enantiomeric separation of eigh t chromane compounds or analogues using capillary electrophoresis. It is shown that the cyclodextrin type and concentration have a large inf luence on the enantiomeric separation obtained for these compounds. A chiral resolution of 1.4 or better could be obtained for all the subst ances with either substituted heptakis(2,3,6-tri-O-methyl)-beta-cyclod extrin or unsubstituted gamma-cyclodextrin as the chiral selector. The influence of the gamma-cyclodextrin concentration, ionic strength and pH on the chiral separations was also investigated with a multivariat e screening design. The detection limit and resolution of the present method allow determinations of the investigated compounds down to a ch iral impurity of less than 0.1 % (area/area).