C-REL AND P65 SUBUNITS BIND TO AN UPSTREAM NF-KAPPA-B SITE IN HUMAN GRANULOCYTE MACROPHAGE-COLONY-STIMULATING FACTOR PROMOTER INVOLVED IN PHORBOL ESTER RESPONSE IN 5637 CELLS

To further clarify the complex transcriptional regulation of the human GM-CSF gene, which was extensively investigated in activated T cells, we have studied the role of an upstream NF-kappa B like site in the 5 637 non-lymphoid cell line, which derives from a bladder carcinoma and constitutively produces GM-CSF. This sequence, named the A element, h as an active role on GM-CSF transcription and is responsive to the tum or promoter PMA in transient transfection experiments. We describe her e a heterodimeric binding complex of NF-kappa B subunits (c-Rel and p6 5) which is identical to the one obtained using the HIV-LTR-kappa B si te as recognition sequence and different from the one (c-Rel and p50) observed with nuclear extracts from Mo T-lymphoid HTLV-II infected cel ls. (C) 1997 Federation of European Biochemical Societies.