STUDIES ON SYNTHESIS AND CHROMATOGRAPHIC PROPERTIES OF NAD COVALENTLYBOUND ONTO PHOSPHOLIPID-COATED AMINATED SILICA

Recently affinity chromatography with a biospecific stationary phase w as widely used in separation and purification processes of all kinds o f enzymes. in this paper, a series of synthetic reactions of solid-liq uid phase on a silica surface are described. By using a wide-pore (30 mu m), microspheric silica (8 mu m) as the matrix and gamma-aminopropy ltriethoxy-silane as the activating agent, the nicotinamide adenine di nucleotide (NAD) was bonded through its amino groups with carboxylic g roups of linked phospholipid by a covalent bond on the aminated suppor ts. This bonded stationary phase provided high thermal stability which could be used for separating nucleotides with good resolution. At the same time, the effects of pH, organic modifier and ionic strength on the retention properties of nucleotides were also investigated.