BUFFER MAY BE THE CRITICAL FACTOR IN MEASUREMENT OF ANTI-PROTHROMBIN ANTIBODY ON A GAMMA-RAY-IRRADIATED PLATE BY ENZYME-LINKED-IMMUNOSORBENT-ASSAY

Authors
MATSUDA J SAITOH N TSUKAMOTO M GOTOH M GOHCHI K KAWASUGI K
Citation
J. Matsuda et al., BUFFER MAY BE THE CRITICAL FACTOR IN MEASUREMENT OF ANTI-PROTHROMBIN ANTIBODY ON A GAMMA-RAY-IRRADIATED PLATE BY ENZYME-LINKED-IMMUNOSORBENT-ASSAY, American journal of hematology, 53(4), 1996, pp. 242-244
Citations number
7
Categorie Soggetti
Hematology
Journal title
American journal of hematology → ACNP
ISSN journal
03618609
Volume
53
Issue
4
Year of publication
1996
Pages
242 - 244
Database
ISI
SICI code
0361-8609(1996)53:4<242:BMBTCF>2.0.ZU;2-G
Abstract
We investigated the influence of different buffers (Tris-buffer and ph osphate buffered saline (PBS)/Tween-20 buffer) on anti-prothrombin ant ibody (aPT) measurement by enzyme-linked immunosorbent assay (ELISA), employing a gamma-ray-irradiated plate. We found considerable discrepa ncies in aPT positivity between each buffer, and we suggest that the u se of Tris-buffer is not suitable for aPT measurement with a gamma-ray -irradiated plate to measure aPT. (C) 1996 Wiley-Liss, Inc.