S. Keay et al., POLYMERASE CHAIN-REACTION AMPLIFICATION OF BACTERIAL 16S RIBOSOMAL-RNA GENES IN INTERSTITIAL CYSTITIS AND CONTROL PATIENT BLADDER BIOPSIES, The Journal of urology, 159(1), 1998, pp. 280-283
Purpose: Several characteristics of the chronic bladder disease called
interstitial cystitis (IC) suggest an infectious etiology. However, a
single causative organism has not been convincingly cultured in vitro
, and DNA for a variety of microorganisms has been found inconsistentl
y in bladder biopsies from IC patients. We therefore looked for a poss
ible bacterial cause for IC by using a sensitive nested PCR assay on c
ystoscopic bladder biopsy specimens obtained from IC patients and cont
rols. Materials and Methods: Bladder biopsies were obtained at cystosc
opy from 6 IC patients and 6 controls. DNA was extracted from these sp
ecimens and PCR with 2-round amplification performed using nested prim
ers from a highly conserved region of the bacterial 16s rRNA gene. Amp
lified DNA was purified and sequenced using the Sequenase PCR Product
Sequencing Kit, and the sequences obtained were compared with bacteria
l rRNA gene sequences recorded in GenBank. Results: Biopsy specimens f
rom all 6 patients and 6 controls were positive by PCR for DNA encodin
g bacterial 16s rRNA. Sequence data indicated a predominant microorgan
ism in 10 of the 12 specimens, with >95% homology to DNA from several
different genera of bacteria including Acinetobacter, Propionobacteriu
m, Salmonella, and Escherichia. None of the organisms identified by PC
R had been cultured from tissue or urine obtained simultaneously from
these persons, using sensitive culture techniques. Conclusions: These
data indicate no difference between IC patients and controls in the pr
oportion of bladder biopsies with PCR positivity or the type(s) of org
anism present, providing additional evidence that IC is not associated
with infection by a particular type of bacterium.