MUTATIONS AT CODON-184 IN SIMIAN IMMUNODEFICIENCY VIRUS REVERSE-TRANSCRIPTASE CONFER RESISTANCE TO THE (-) ENANTIOMER OF 2',3'-DIDEOXY-3'-THIACYTIDINE

Authors
CHERRY E SLATER M SALOMON H RUD E WAINBERG MA
Citation
E. Cherry et al., MUTATIONS AT CODON-184 IN SIMIAN IMMUNODEFICIENCY VIRUS REVERSE-TRANSCRIPTASE CONFER RESISTANCE TO THE (-) ENANTIOMER OF 2',3'-DIDEOXY-3'-THIACYTIDINE, Antimicrobial agents and chemotherapy, 41(12), 1997, pp. 2763-2765
Citations number
24
Journal title
Antimicrobial agents and chemotherapy → ACNP
ISSN journal
00664804
Volume
41
Issue
12
Year of publication
1997
Pages
2763 - 2765
Database
ISI
SICI code
0066-4804(1997)41:12<2763:MACISI>2.0.ZU;2-#
Abstract
Variants of simian immunodeficiency virus (SIV) that display greater t han 2,000-fold resistance to the (-) enantiomer of 2',3'-dideoxy-3'-th iacytidine (3TC) were generated through in vitro passage and drug sele ction. The polymerase regions of several of these resistant viruses we re sequenced and were found to share either of two codon alterations a t site 184 in reverse transcriptase (ATG to ATA [methionine to isoleuc ine] and ATG to GTA [methionine to valine]). The biological relevance of these substitutions for 3TC was confirmed by site-directed mutagene sis with the SIVmac239 infectious recombinant clone of SIV.