Mj. Bennett et al., CATIONIC LIPID-MEDIATED GENE DELIVERY TO MURINE LUNG - CORRELATION OFLIPID HYDRATION WITH IN-VIVO TRANSFECTION ACTIVITY, Journal of medicinal chemistry, 40(25), 1997, pp. 4069-4078
A panel of lipidic tetraalkylammonium chlorides has been prepared and
screened in studies of both lipid hydration and in vivo mouse transfec
tion. The effect of cationic lipid structure on liposome surface hydra
tion was determined using differential scanning calorimetry. Increases
in headgroup steric bulk and the inclusion of cis-unsaturation in the
hydrophobic domain led to greater lipid hydration, indicative of a de
crease in lipid polar domain associations. Cationic lipids containing
hydrogen-bonding functionality in the polar domain exhibited a corresp
onding decrease in observed lipid hydration, indicative of an increase
in lipid polar domain associations. To explore a potential correlatio
n of the hydration data with transfection activity, we examined the in
vivo transfection activity of the lipid panel by direct intratracheal
instillation of cationic liposome-DNA complexes into BALB/c mice. The
more active transfection agents were the lipids that featured headgro
up structures promoting close polar domain association in combination
with fatty acyl cis-unsaturation. The hydration data suggest that the
more effective transfection lipids for mouse lung delivery are those p
ossessing the greatest imbalance between the cross-sectional areas occ
upied by the polar and hydrophobic domains.