INTEGRATED RADIATION HYBRID AND YEAST ARTIFICIAL CHROMOSOME MAP OF CHROMOSOME 9P

A panel of 93 radiation-reduced hybrids have been screened using PCR a mplification and oligonucleotide primers for sequence-tagged sites (ST Ss) specific for 114 single-copy loci mapping to the short arm of chro mosome 9. An x-ray dose of 6,000 rads gave an average retention freque ncy of approximately 23%. We have constructed a framework map containi ng 31 markers ordered by analyzing coretention patterns, with support for the order greater than 1,000:1. In addition, we have placed the re maining markers which could not be mapped to a single interval with th is support to a range of intervals on the framework map. The STS oligo nucleotide primers used in the construction of the radiation hybrid (R H) map have been used to isolate and order yeast artifical chromosomes (YACs) assigned to 9p identified from the CEPH mega-YAC library. Eigh ty-nine STS markers have screened positive with at least one YAC. A to tal of 88 individual YACs (with an average size of 0.9 MB) have been p laced on the map in a series of contigs and in some cases mapped cytog enetically by fluorescence in situ hybridization. Additionally, the YA C information has been used in conjunction with the RH framework place ments to generate an integrated map containing 65 loci including 51 un iquely positioned markers, with an average resolution of 0.79 Mb.