SUBLOCALIZATION OF THE BREAKPOINTS OF A T(5-16) IN MYELODYSPLASIA

As a first step in characterizing a t(5;16)(q31;p11.2) in a patient wi th the diagnosis refractory anemia with ring sideroblasts, a cell fusi on was carried out between bone marrow cells from the patient and the Chinese hamster cell line A3. Using PCR and FISH analysis on hybrid li nes containing the human derivative 16 chromosome, the breakpoints cou ld be mapped between the markers TCF-7 and IL-9 on chromosome 5 and OL -7 and s30A4 on chromosome 16, both regions spanning approximately 1 M b. Since the breakpoint on 5q has occurred in a region that is frequen tly deleted in myeloid malignancies, the gene disrupted by this transl ocation could also be implicated in this aberration. (C) Elsevier Scie nce Inc., 1998.