NEURONS IN PRIMARY CULTURE ARE LESS EFFICIENTLY INFECTED BY TOXOPLASMA-GONDII THAN GLIAL-CELLS

Infection by Toxoplasma gondii is asymptomatic, leading to an immune r esponse that controls the disease. In immune-compromised patients, how ever, quiescent cysts can reactivate, leading to toxoplasmic encephali tis. We studied the infection of cells of the central nervous system i n an attempt to understand the process leading to this complication. P rimary cultures of rat hippocampal glial cells and neurons were infect ed with the virulent strain RH and examined by immunofluorescent micro scopy after fixation of cells and staining with antibodies specific to the different cell types. After 24 h of infection, glial cells were h ighly infected and showed active division of the parasite. Neurons, on the other hand, were much less efficiently infected than glial cells, but actual penetration of the parasites was demonstrated by confocal microscopy. Whereas glial cells contained vacuoles with several parasi tes, the vacuoles observed in neurons usually contained one parasite o r. rarely, two, indicating that the parasites inside neurons did not u ndergo active division. This was corroborated by determination of the incorporation of [H-3]-uracil. Little is known about the mechanism of neuronal infection by Toxoplasma. The experimental setup used in this study should help to improve our understanding of neuronal infection a nd bring insight into the physiopathology of toxoplasmic encephalitis.