A direct agglutination test was evaluated for the detection and quanti
tation of IgG antibodies to Neospora caninum in both experimental and
natural infections in various animal species. As compared with results
obtained by the indirect fluorescent antibody test, the direct agglut
ination test appeared reliable for the serologic diagnosis of neosporo
sis in a variety of animal species. The direct agglutination test shou
ld provide easily available and inexpensive tools for serologic testin
g for antibodies to N. caninum in many host species.