IDENTIFICATION OF NEW TRANSLOCATION BREAKPOINTS AT 12Q13 IN LIPOMAS

Cytogenetic studies of banded chromosomes and fluorescence in situ hyb ridization (FISH) of several yeast artificial chromosomes (YACs) that are part of a 128-kb resolution physical map of a portion of 12q13 rev ealed that 4/14 (28%) lipomas have breakpoints in 12q13. These breakpo ints are more than 10 Mb away from the HMGIC gene at 12q14-q15, which is known to be modified in some lipomas. FISH with individual YACs at 12q13 enabled us to identify four YACs that span three breakpoints. Ou r results suggest Chat genes other than HMGIC on human chromosome 12 m ay be involved in the etiology of lipoma development. (C) 1997 Academi c Press.