A. Molina et al., DIFFERENTIAL EXPRESSION OF PATHOGEN-RESPONSIVE GENES ENCODING 2 TYPESOF GLYCINE-RICH PROTEINS IN BARLEY, Plant molecular biology, 33(5), 1997, pp. 803-810
Gene-specific probes (3' ends of cDNAs) were obtained from barley cDNA
s encoding two types of glycine-rich proteins: HvGRP2, characterized b
y a cytokeratin-like and a cysteine-rich domain, and HVGRP3, whose mai
n feature was an RNA-binding domain. Expression of genes Hvgrp2 and Hv
grp3, which are present at one (or two) copies per haploid genome, was
ubiquitous and gene Hvgrp3 was under light/darkness modulation. Cold
treatment increased Hvgrp2 and Hvgrp3 mRNA levels. Methyl jasmonate (1
0 mu M) switched off the two genes. Expression of Hvgrp2, but not that
of Hvgrp3, was induced by ethylene treatment (100 ppm). Fungal pathog
ens Erysiphe graminis and Rhynchosporium secalis increased the mRNAs l
evels of the two genes, both in compatible and in incompatible interac
tions, while bacterial pathogens did not.