COMPARISON OF THE ACTIVITIES OF CAMV 35S AND FMV 34S PROMOTER DERIVATIVES IN CATHARANTHUS-ROSEUS CELLS TRANSIENTLY AND STABLY TRANSFORMED BY PARTICLE BOMBARDMENT

Citation
L. Vanderfits et J. Memelink, COMPARISON OF THE ACTIVITIES OF CAMV 35S AND FMV 34S PROMOTER DERIVATIVES IN CATHARANTHUS-ROSEUS CELLS TRANSIENTLY AND STABLY TRANSFORMED BY PARTICLE BOMBARDMENT, Plant molecular biology, 33(5), 1997, pp. 943-946
Citations number
17
Categorie Soggetti
Plant Sciences",Biology
Journal title
ISSN journal
01674412
Volume
33
Issue
5
Year of publication
1997
Pages
943 - 946
Database
ISI
SICI code
0167-4412(1997)33:5<943:COTAOC>2.0.ZU;2-8
Abstract
Activities of several CaMV 35S and FMV 34S promoter derivatives fused to the gusA reporter gene were compared in suspension-cultured Cathara nthus roseus cells that were transiently and stably transformed using particle bombardment. Our data demonstrate that the 35S and a deletion derivative of the 34S promoter combined with particle bombardment for m useful tools for genetic engineering of C. roseus cells. Our results disagree on several points with activities of 35S and 34S promoter de rivatives reported for tobacco, indicating that absolute and relative promoter activities can differ between plant species.