TISSUE SUSPENSION AGGLUTINATION - A SIMPLE METHOD TO SCREEN SPECIES-SPECIFIC AND ORGAN-SPECIFIC REACTIONS

Agglutination tests with preparations of parenchymatous organs were de veloped. The tissue suspensions were dried at room temperature after t hey had been spread as a very thin layer on a glass plate, or otherwis e, they were lyophilized. The dried preparations were pulverized and t hen prepared as stable suspensions in saline. The agglutination test w as conducted on a slide by mixing one drop of the tested serum at a co nvenient dilution with one drop of tissue powder suspension. Agglutina tion in the form of readily discernible clumps could be assessed after 1-10 min. By means of this procedure, species-specific reactions were studied using suspensions of kidneys of various species. Organ-specif ic reactions were noted with suspensions of brain and thyroid. Aggluti nation of thyroid powder was observed with rabbit anti-rabbit thyroid sera as well as with many, albeit not all, sera of patients with Hashi moto's disease.