IMMUNOCHEMICAL ANALYSIS OF LARVAL ANTIGENS OF TRICHINELLA-SPIRALIS AND TRICHINELLA-PSEUDOSPIRALIS

Authors
TURCEKOVA L BOROSKOVA Z TOMASOVICOVA O REITEROVA K KINCEKOVA J
Citation
L. Turcekova et al., IMMUNOCHEMICAL ANALYSIS OF LARVAL ANTIGENS OF TRICHINELLA-SPIRALIS AND TRICHINELLA-PSEUDOSPIRALIS, Helminthologia, 34(4), 1997, pp. 241-243
Citations number
14
Journal title
Helminthologia → ACNP
ISSN journal
04406605
Volume
34
Issue
4
Year of publication
1997
Pages
241 - 243
Database
ISI
SICI code
0440-6605(1997)34:4<241:IAOLAO>2.0.ZU;2-9
Abstract
Antigens were isolated from larvae and excretory/secretory products of Trichinella spiralis and T. pseudospiralis. Both antigens were analys ed using Western blot. In somatic T. spiralis larvae antigens were ide ntified bands with 18, 43, 47, 65, 80 and 94 kDa whereas bands 65 and 80 kDa were not identified in antigen T. pseudospiralis. An analysis w ith the immunoglobulins isolated from the hyperimmune serum revealed t he presence of six bands ranging from 43-70 kDa in T. spiralis and 3 b and (43, 48 and 70 kDa) in T. pseudospiralis. The number of excretory/ secretory fractions was dependent on the length of incubation larvae o f T. spiralis. The results are significant for the immunodiagnosis of larval trichinellosis.