The 1,2-kb DNA sequence flanking the transcription start of the AdoHcy
hydrolase gene was cloned into the luciferase reporter plasmid pGL3-b
asic, and promoter activity was measured in transiently transfected CH
O cells. Deletion analysis showed that most promoter activity was loca
ted within a 153 bp fragment immediately upstream from the predominant
transcription start. The 153 bp fragment includes sites for AP-2, glu
cocorticoid-responsive element, SP-1, and a TATA-like sequence TATTTAA
A, Mutational analysis demonstrated that the SP-1 site nearest the sta
rt of transcription contributed significantly to promoter activity, wh
ereas, the other elements, including the appropriately positioned TATT
TAAA sequence, had little affect on promoter activity. (C) 1997 Academ
ic Press.