THE RAT S-ADENOSYLHOMOCYSTEINE HYDROLASE PROMOTER

The 1,2-kb DNA sequence flanking the transcription start of the AdoHcy hydrolase gene was cloned into the luciferase reporter plasmid pGL3-b asic, and promoter activity was measured in transiently transfected CH O cells. Deletion analysis showed that most promoter activity was loca ted within a 153 bp fragment immediately upstream from the predominant transcription start. The 153 bp fragment includes sites for AP-2, glu cocorticoid-responsive element, SP-1, and a TATA-like sequence TATTTAA A, Mutational analysis demonstrated that the SP-1 site nearest the sta rt of transcription contributed significantly to promoter activity, wh ereas, the other elements, including the appropriately positioned TATT TAAA sequence, had little affect on promoter activity. (C) 1997 Academ ic Press.