HYDROXY JUVENILE HORMONES - NEW PUTATIVE JUVENILE HORMONES BIOSYNTHESIZED BY LOCUST CORPORA ALLATA IN-VITRO

The in vitro production of sesquiterpenoids was investigated by using corpora allata (CA) of the African locust Locusta migratoria migratori oides. Labeled products from unstimulated biosynthesis were extracted, purified by normal phase HPLC, and derivatized to determine the funct ional groups present. An extra hydroxyl group was detected in each of two juvenile hormone (JH) biosynthetic products. One compound, NP-8, w as found to co-migrate with a chemically-synthesized (Z)-hydroxymethyl isomer, 12'-OH JH-III, but not with the (E)-hydroxymethyl isomer, 12- OH JH III, Mass spectral analyses further supported the identity of th e synthetic material with that biosynthesized by the corpora allata. A second compound was identified as the 8'-OH JH-III based on spectrosc opic analyses, 12'-OH JH-III exhibited morphogenetic activity when tes ted on the heterospecific Tenebrio test. These data suggest that 12'-O H JH-III and 8'-OH JH-III are additional biosynthetically-produced and biologically-active juvenile hormones, and constitute the first known members of the class of hydroxy juvenile hormones (HJHs). (C) 1997 Ac ademic Press.