MOSQUITO LARVICIDAL ACTIVITY OF TRANSGENIC ANABAENA STRAIN PCC-7120 EXPRESSING COMBINATIONS OF GENES FROM BACILLUS-THURINGIENSIS SUBSP ISRAELENSIS

Authors
WU XQ VENNISON SJ LIU HR BENDOV E ZARITSKY A BOUSSIBA S
Citation
Xq. Wu et al., MOSQUITO LARVICIDAL ACTIVITY OF TRANSGENIC ANABAENA STRAIN PCC-7120 EXPRESSING COMBINATIONS OF GENES FROM BACILLUS-THURINGIENSIS SUBSP ISRAELENSIS, Applied and environmental microbiology, 63(12), 1997, pp. 4971-4974
Citations number
33
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
63
Issue
12
Year of publication
1997
Pages
4971 - 4974
Database
ISI
SICI code
0099-2240(1997)63:12<4971:MLAOTA>2.0.ZU;2-M
Abstract
Various combinations of the genes cryIVA (cry4A), cryIVD) (cry11A), an d p20 from Bacillus thuringiensis subsp. israelensis were introduced i nto the nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120 by means of Escherichia coli-Anabaena shuttle vector pRL488p and were ex pressed under control of two tandem strong promoters, a cyanobacterial promoter (PpsbA) and an E. coli T7 promoter (P-A1). Two of the clones carrying cryIVA plus cryIVD, one with p20 and one without p20, displa yed toxicity against third-instar larvae of Aedes aegypti at levels gr eater than any level previously reported for transgenic cyanobacteria.