ONE-STEP PURIFICATION OF NISIN-A BY IMMUNOAFFINITY CHROMATOGRAPHY

Authors
SUAREZ AM AZCONA JI RODRIGUEZ JM SANZ B HERNANDEZ PE
Citation
Am. Suarez et al., ONE-STEP PURIFICATION OF NISIN-A BY IMMUNOAFFINITY CHROMATOGRAPHY, Applied and environmental microbiology, 63(12), 1997, pp. 4990-4992
Citations number
21
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
63
Issue
12
Year of publication
1997
Pages
4990 - 4992
Database
ISI
SICI code
0099-2240(1997)63:12<4990:OPONBI>2.0.ZU;2-N
Abstract
The lantibiotic nisin A was purified to homogeneity by a single-step i mmunoaffinity chromatography method. An immunoadsorption matrix was de veloped by direct binding of anti-nisin A monoclonal antibodies to N-h ydroxysuccinimide-activated Sepharose. The purification procedure was rapid and reproducible and rendered much higher final yields of nisin than any other described method.