Mc. Polanco et Ml. Ruiz, EFFECT OF BENZYLAMINOPURINE ON IN-VITRO AND IN-VIVO ROOT DEVELOPMENT IN LENTIL, LENS-CULINARIS MEDIK, Plant cell reports, 17(1), 1997, pp. 22-26
The effect of benzylaminopurine (BAP) on the formation of roots from l
entil shoots regenerated on media containing BAP was studied. Seedling
shoot tips, first nodes and bractlets, and immature seeds cultured on
the initiation media containing 2.25 or 0.225 mg/l of BAP regenerated
multiple bud shoots. The regenerated shoots formed roots in percentag
es ranging from 4.6 to 39.9% on a rooting medium (R medium) containing
2 mg/l of indoleacetic acid. Rooting success on R medium depended upo
n the cytokinin used in the initiation media, its concentration, and t
he time elapsed during shoot formation on these media prior to transpl
anting regenerated shoots to R medium. In vivo study of root growth of
lentil seedlings demonstrated the strong inhibitory effect of BAP on
root growth reflected in a drastic reduction of the mitotic index of t
he root meristem.