INTERLEUKIN-10 IS EXPRESSED IN HUMAN GLIOMAS IN-VIVO AND INCREASES GLIOMA CELL-PROLIFERATION AND MOTILITY IN-VITRO

Citation
C. Huettner et al., INTERLEUKIN-10 IS EXPRESSED IN HUMAN GLIOMAS IN-VIVO AND INCREASES GLIOMA CELL-PROLIFERATION AND MOTILITY IN-VITRO, Anticancer research, 17(5A), 1997, pp. 3217-3224
Citations number
30
Categorie Soggetti
Oncology
Journal title
ISSN journal
02507005
Volume
17
Issue
5A
Year of publication
1997
Pages
3217 - 3224
Database
ISI
SICI code
0250-7005(1997)17:5A<3217:IIEIHG>2.0.ZU;2-U
Abstract
Interleukin 10 (IL-IO) is a cytokine with a broad spectrum of immunosu ppressive activity, but it's role in the oncogenesis of solid tumors i s still unclear: In previous experiments we have shown that IL-IO spec ific mRNA is produced within glial rumors in vivo. The aim of the pres ent study was to investigate the expression of the TL-IO protein in vi vo and to identify the cells producing IL-10 within the tumor tissue. Expression levels significantly increased with malignancy of the gliom as. 87.5% of grade III and IV, but only 4% of grade II tumors expresse d high levels of mRNA. Elevation of IL-IO serum levels was found in 11 % of low grade and in 63,6% of high grade glioma patients. In situ hyb ridization analysis with combined immunohistochemistry revealed that. a) IL-10 is not produced by infiltrating B-or T-lymphocytes, b) both m icroglia and astroglia contributed to IL-10 expression in malignant gl iomas in vivo. These data suggested the functional role of IL10 in gli oma progression. Therefore, the effects of IL-10 on proliferation and migration of glioma cells were determined in vitro. Two human glioma c ell lines were grown as monolayer as well as spheroids in the presence of different concentrations of IL-10. IL-10 increased cell proliferat ion significantly in both culture systems with a dose optimum of 25 ng /ml. Glioma cell motility was enhanced with 25 ng/ml as the optimal do se. Adding the IL-10 specific antibody reversed both effects. We concl ude from our data that IL-IO is involved in the progression of glial t umors, especially in the enhancement of tumor cell proliferation and m igration which promotes infiltration of the surrounding tissue.