GROWTH-INHIBITION OF A HUMAN LUNG ADENOCARCINOMA CELL-LINE BY GENETICCOMPLEMENTATION WITH CHROMOSOME-11

Two regions on chromosome segment 11p15.5 have frequent allele loss in lung cancer. LOH11A is centromeric between loci D11S1758 and D11S12, and LOH11B is telomeric between HRAS and D11S1363. We studied the biol ogical significance of this allele loss using microcell-mediated trans fer of human chromosomes 11, 11p, and two radiation-reduced fragments of 11p into human lung adenocarcinoma cell lines. Chromosome 12, which has not been implicated in lung carcinogenesis, was used as a control . All four chromosome 11-containing hybrid clones showed significantly reduced tumorigenicity in nude mice and growth in liquid culture. The se findings support the notion of a tumor suppressor gene located in t he LOH11A region on chromosome segment 11p15.5.