ISOLATION OF A CDNA CLONE FROM COLON-CARCINOMA

cDNA clones of differentially expressed mRNAs in a colon carcinoma hav e been isolated by subtractive cDNA cloning. The subtracted material w as at least 90x enriched for differentially expressed sequences and ca n be used for the construction of subtractive cDNA libraries and polym erase chain reaction (PCR) amplification to generate differential prob es. In this way rare mRNA (less than 0.1% abundance) which are differe ntially expressed, can be isolated utilising this procedure. A cDNA cl one which we call IH12 has been isolated. Its mRNA is expressed exclus ively in actively proliferating cells.