CONFORMATIONAL-CHANGES IN GASTRIC-CARCINOMA CELL-MEMBRANE PROTEIN CORRELATED TO CELL VIABILITY AFTER TREATMENT WITH ADAMANTYL MALEIMIDE

Background: In order to investigate the mechanism of the effects of th e anticancer drug adamantyl maleimide (AMI) on the in vitro growth of a human gastric carcinoma cell line (SC-MI), the membrane protein conf ormation on the cell surface after treatment with AMI was studied. Mat erials and Methods: The interaction of AMI with SC-MI cell line was st udied by reflectance Fourier transform infrared (FT-IR) microspectrosc opy. The cell viability was also determined by the trypan blue exclusi on method Results: FT-IR microscopic study provided evidence that the conformational conversion of the membrane proteins of the SC-MI cell l ine from alpha-helix to beta-sheet was found evident, but was dependen t on the AMI concentrations used and the time of treatment. AMI nor on ly interacted with the membrane protein on the cell surface but also e ntered into cells to interact with the nucleic acids. Conclusions: The relationship between membrane protein conformational changes and the cell viability of SC-M1 cell line was evident. The AMI-induced apoptos is in SC-M1 cell line was also supposed.